Difference between revisions of "GltC"

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=References=
 
=References=
 
==Reviews==
 
==Reviews==
<pubmed> 20408793 </pubmed>
+
<pubmed> 20408793 22625175 </pubmed>
 
 
 
==Original Publications==
 
==Original Publications==
 
<pubmed>7559360 15150225 2548995 17183217 17608797 17134717 14523131, 20630473</pubmed>
 
<pubmed>7559360 15150225 2548995 17183217 17608797 17134717 14523131, 20630473</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:05, 28 May 2012

  • Description: Transcriptional activator of the gltA-gltB operon. Activates expression of the operon in the absence of arginine.

Gene name gltC
Synonyms
Essential No
Product transcriptional regulator (LysR family)
Function positive regulation
of the glutamate synthase operon (gltAB)
Interactions involving this protein in SubtInteract: GltC
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 33.9 kDa, 5.62
Gene length, protein length 900 bp, 300 amino acids
Immediate neighbours gltA, proJ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GltC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GltC expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, transcription factors and their control

This gene is a member of the following regulons

The GltC regulon:

The gene

Basic information

  • Locus tag: BSU18460

Phenotypes of a mutant

gltC mutants are auxotrophic for glutamate.

Database entries

  • DBTBS entry: [1]
  • SubtiList entry:[2]

Additional information

Expression

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcription activation of the gltA-gltB operon PubMed
  • Paralogous protein(s): none, but there are 19 members of the LysR family in B. subtilis

Extended information on the protein

  • Kinetic information:
  • Domains: DNA-binding helix-turn-helix motif: AA 18 ... 37
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: 2-oxoglutarate stimulates transcription activation, glutamate inhibits transcription activation PubMed
  • Interactions:
    • active as dimer
    • GltC-RocG, This interaction takes place in the presence of glutamate. It prevents the transcription activation of the gltA-gltB operon. Note that RocG expression is strongly regulated by carbon and nitrogen sources, respectively. PubMed

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: autoregulation by GltC PubMed
  • Regulatory mechanism: autorepression PubMed
  • Additional information:

Biological materials

  • Mutant: GP344 (erm), GP738 (spc) (available in Stülke lab)
  • Expression vector:
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP903, available in Stülke lab
    • for expression, purification in E. coli with C-terminal Strep-tag, in pET3C: pGP951, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody: available in Stülke lab

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Jörg Stülke, University of Göttingen, Germany Homepage

Fabian Commichau University of Göttingen, Germany Homepage

Your additional remarks

References

Reviews

Katrin Gunka, Fabian M Commichau
Control of glutamate homeostasis in Bacillus subtilis: a complex interplay between ammonium assimilation, glutamate biosynthesis and degradation.
Mol Microbiol: 2012, 85(2);213-24
[PubMed:22625175] [WorldCat.org] [DOI] (I p)

Sabine Brantl, Andreas Licht
Characterisation of Bacillus subtilis transcriptional regulators involved in metabolic processes.
Curr Protein Pept Sci: 2010, 11(4);274-91
[PubMed:20408793] [WorldCat.org] [DOI] (I p)

Original Publications