Difference between revisions of "SigL"

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(Original publications)
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* '''Mutant:''' GP146 (aphA3), available in [[Stülke]] lab
 
* '''Mutant:''' GP146 (aphA3), available in [[Stülke]] lab
 +
** 1A914 ( ''sigL''::''kan''), {{PubMed|16585774}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A914&Search=1A914 BGSC]
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 12:12, 19 September 2012

Gene name sigL
Synonyms
Essential no
Product RNA polymerase sigma-54 factor (sigma-L)
Function utilization of arginin, acetoin and fructose,
required for cold adaptation
Gene expression levels in SubtiExpress: sigL
Interactions involving this protein in SubtInteract: SigL
MW, pI 49,5 kDa, 7.79
Gene length, protein length 1308 bp, 436 amino acids
Immediate neighbours yvfH, yvfG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SigL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SigL expression.png

























Categories containing this gene/protein

transcription, sigma factors and their control

This gene is a member of the following regulons

CcpA regulon

The SigL regulon

The gene

Basic information

  • Locus tag: BSU34200

Phenotypes of a mutant

The mutant is cold-sensitive and unable to use arginine as a single carbon source. PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binding to promoters of the -12, -24 type
  • Protein family: sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family
  • Paralogous protein(s):

Transcription factors activating transcription at SigL-dependent promoters

AcoR, BkdR, LevR, RocR, YplP

Extended information on the protein

  • Kinetic information:
  • Domains:
    • DNA binding domain (H-T-H motif) (324–343)
    • pron box domain (413–421)
    • 3 x Compositional bias domain (6–21),(32–53),(112–136)
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Transcription initiation by SigL-containing RNA polymerase requires the activity of ATP-hydrolyzing transcription activators.

Transcription factors activating transcription at SigL-dependent promoters

Expression and regulation

  • Operon: sigL (according to DBTBS)
  • Sigma factor:
  • Regulatory mechanism: CcpA: transcription repression, transcriptional roadblock PubMed
  • Additional information:

Biological materials

  • Mutant: GP146 (aphA3), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage

Your additional remarks

References

Reviews

Additional reviews: PubMed

M Buck, M T Gallegos, D J Studholme, Y Guo, J D Gralla
The bacterial enhancer-dependent sigma(54) (sigma(N)) transcription factor.
J Bacteriol: 2000, 182(15);4129-36
[PubMed:10894718] [WorldCat.org] [DOI] (P p)

M J Merrick
In a class of its own--the RNA polymerase sigma factor sigma 54 (sigma N).
Mol Microbiol: 1993, 10(5);903-9
[PubMed:7934866] [WorldCat.org] [DOI] (P p)


Original publications