Difference between revisions of "MaeN"

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(Biological materials)
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** 1A642 (''maeN''::Tn917, erm) (available from the [http://www.bgsc.org/ BGSC] or in [[Jörg Stülke]]'s lab)
 
** 1A642 (''maeN''::Tn917, erm) (available from the [http://www.bgsc.org/ BGSC] or in [[Jörg Stülke]]'s lab)
 
** 1A642 ( ''maeN''::''erm''), {{PubMed|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A642&Search=1A642 BGSC]
 
** 1A642 ( ''maeN''::''erm''), {{PubMed|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A642&Search=1A642 BGSC]
 +
** GP1449 (''erm''), available in [[Stülke]] lab
  
 
* '''Expression vector:'''  
 
* '''Expression vector:'''  

Revision as of 08:23, 2 November 2012

  • Description: Na+/malate symporter

Gene name maeN
Synonyms yufR
Essential no
Product Na+/malate symporter
Function malate uptake
Gene expression levels in SubtiExpress: maeN
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 47 kDa, 8.411
Gene length, protein length 1344 bp, 448 aa
Immediate neighbours nupQ, yufS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MaeN context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MaeN expression.png




























Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

MalR regulon

The gene

Basic information

  • Locus tag: BSU31580

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: sodium:citrate (SCF) symporter family (according to Swiss-Prot) 2-hydroxycarboxylate transporter family
  • Paralogous protein(s): CimH

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: induced by malate (MalR)
  • Regulatory mechanism: MalR: transcription activation
  • Additional information:
    • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

  • Mutant:
  • Expression vector:
    • expression of native maeN in B. subtilis: pGP1951 (in pBQ200), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947