CarA

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  • Description: carbamoyl-phosphate transferase-arginine (subunit A)

Gene name carA
Synonyms cpa
Essential no
Product carbamoyl-phosphate transferase-arginine (subunit A)
Function biosynthesis of arginine
Metabolic function and regulation of this protein in SubtiPathways:
Pyrimidines, Ammonium/ glutamate
MW, pI 38 kDa, 6.212
Gene length, protein length 1059 bp, 353 aa
Immediate neighbours argD, carB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CarA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU11230

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate (according to Swiss-Prot)
  • Protein family: glutamine amidotransferase type-1 domain (according to Swiss-Prot)
  • Paralogous protein(s): PyrAA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: repressed by casamino acids PubMed, repressed by arginine (AhrC)
  • Regulatory mechanism: AhrC: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

A Mountain, N H Mann, R N Munton, S Baumberg
Cloning of a Bacillus subtilis restriction fragment complementing auxotrophic mutants of eight Escherichia coli genes of arginine biosynthesis.
Mol Gen Genet: 1984, 197(1);82-9
[PubMed:6096675] [WorldCat.org] [DOI] (P p)