Ldh

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  • Description: L-lactate dehydrogenase

Gene name ldh
Synonyms lctE
Essential no
Product L-lactate dehydrogenase
Function overflow metabolism, fermentation
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 34 kDa, 5.394
Gene length, protein length 963 bp, 321 aa
Immediate neighbours amyE, lctP
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Ldh context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU03050

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-lactate + NAD+ = pyruvate + NADH (according to Swiss-Prot)
  • Protein family: LDH family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Tyr-224 PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 1LDN (+) and fructose-1.6-bisphosphate, Geobacillus stearothermophilus), 2LDB (Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced under anaerobic conditions (Rex) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Susana Romero, Enrique Merino, Francisco Bolívar, Guillermo Gosset, Alfredo Martinez
Metabolic engineering of Bacillus subtilis for ethanol production: lactate dehydrogenase plays a key role in fermentative metabolism.
Appl Environ Microbiol: 2007, 73(16);5190-8
[PubMed:17586670] [WorldCat.org] [DOI] (P p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Jonas T Larsson, Annika Rogstam, Claes von Wachenfeldt
Coordinated patterns of cytochrome bd and lactate dehydrogenase expression in Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 10);3323-3335
[PubMed:16207915] [WorldCat.org] [DOI] (P p)