DivIVA

From SubtiWiki
Revision as of 08:37, 27 August 2009 by SHalbedel (talk | contribs) (The protein)
Jump to: navigation, search
  • Description: cell-division initiation protein (septum placement)

Gene name divIVA
Synonyms ylmJ
Essential no
Product cell-division initiation protein
Function septum placement
MW, pI 19 kDa, 4.846
Gene length, protein length 492 bp, 164 aa
Immediate neighbours ylmH, ileS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DivIVA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU15420

Phenotypes of a mutant

Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. A divIVA mutant has a severe sporulation defect.

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

filamentation is suppressed by minCD mutations

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: DivIVA is required for polar localisation of MinCD via MinJ. PubMed It also recruits RacA to the distal pole of the prespore PubMed.
  • Protein family: gpsB family (according to Swiss-Prot)
  • Paralogous protein(s): GpsB

Extended information on the protein

  • Kinetic information:
  • Domains: The first 60 amino acids constitute a lipid binding domain. PubMed

Multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain PubMed.

  • Modification: The Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73 PubMed.
  • Cofactor(s): not known
  • Effectors of protein activity: not known
  • Localization: DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles PubMed.

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: one gene cistron
  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: divIVA::tet available from the Hamoen Lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion: divIVA-gfp fusions available from the Hamoen Lab
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Leendert Hamoen, Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [4]

Imrich Barak, Slovak Academy of Science, Bratislava, Slovakia homepage

Your additional remarks

References