LevE

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Gene name levE
Synonyms
Essential no
Product trigger enzyme: fructose-specific phosphotransferase system, EIIB component
Function fructose uptake and phosphorylation, control of LevR activity
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 17 kDa, 9.444
Gene length, protein length 486 bp, 162 aa
Immediate neighbours levF, levD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LevE context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU27060

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)
  • Protein family: PTS permease, mannose permease (Man) family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation: repressed by glucose (CcpA) , carbon catabolite repression, induction by fructose
  • Regulatory mechanism: CcpA: transcription repression, catabolite repression: transcription repression by CcpA, transcription activator LevR is less active in the presence of glucose; induction: transcription activation by LevR
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References