GlnK

From SubtiWiki
Revision as of 11:58, 20 May 2010 by Heilers (talk | contribs) (Extended information on the protein)
Jump to: navigation, search
Gene name glnK
Synonyms ycbA, yzgA
Essential no
Product two-component sensor kinase
Function regulation of the glsA-glnT operon
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 43 kDa, 5.731
Gene length, protein length 1161 bp, 387 aa
Immediate neighbours glsA, glnL
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YcbA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU02440

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of GlnL
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: four transmembrane segments, C-terminal histidine phosphotransferase domain
  • Modification: autophosphorylation on a His residue
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Annette Heinrich, Kathrin Woyda, Katja Brauburger, Gregor Meiss, Christian Detsch, Jörg Stülke, Karl Forchhammer
Interaction of the membrane-bound GlnK-AmtB complex with the master regulator of nitrogen metabolism TnrA in Bacillus subtilis.
J Biol Chem: 2006, 281(46);34909-17
[PubMed:17001076] [WorldCat.org] [DOI] (P p)

Takenori Satomura, Daisuke Shimura, Kei Asai, Yoshito Sadaie, Kazutake Hirooka, Yasutaro Fujita
Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system.
J Bacteriol: 2005, 187(14);4813-21
[PubMed:15995196] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)