Mta

• Description: transcriptional activator of multidrug-efflux transporter genes, activates ymfD expression
  
  

Gene name	mta
Synonyms	ywnD
Essential	no
Product	transcriptional activator
Function	regulation of multidrug-efflux transporter genes
MW, pI	29 kDa, 4.949
Gene length, protein length	771 bp, 257 aa
Immediate neighbours	clsA, ywnC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transcription factors and their control, resistance against toxins/ antibiotics

This gene is a member of the following regulons

Mta regulon

The gene

Basic information

• Locus tag: BSU36600

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1JBG (N-terminal domain), 1R8D (complex of N-terminal domain with DNA)

• UniProt: P71039

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mta PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • positive autoregulation PubMed

• Regulatory mechanism:
  • Mta: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

Richard Brennan, Houston, Texas, USA Homepage

References

Marcus Miethke, Sarah Schmidt, Mohamed A Marahiel
The major facilitator superfamily-type transporter YmfE and the multidrug-efflux activator Mta mediate bacillibactin secretion in Bacillus subtilis.
J Bacteriol: 2008, 190(15);5143-52
[PubMed:18502870] [WorldCat.org] [DOI] (I p)

Kate J Newberry, Richard G Brennan
The structural mechanism for transcription activation by MerR family member multidrug transporter activation, N terminus.
J Biol Chem: 2004, 279(19);20356-62
[PubMed:14985361] [WorldCat.org] [DOI] (P p)

M H Godsey, N N Baranova, A A Neyfakh, R G Brennan
Crystal structure of MtaN, a global multidrug transporter gene activator.
J Biol Chem: 2001, 276(50);47178-84
[PubMed:11581256] [WorldCat.org] [DOI] (P p)

M H Godsey, N N Baranova, A A Neyfakh, R G Brennan
Crystallization and preliminary X-ray diffraction studies on the DNA-binding domain of the multidrug transporter activation protein (MtaN) from Bacillus subtilis.
Acta Crystallogr D Biol Crystallogr: 2000, 56(Pt 11);1456-8
[PubMed:11053850] [WorldCat.org] [DOI] (P p)

N N Baranova, A Danchin, A A Neyfakh
Mta, a global MerR-type regulator of the Bacillus subtilis multidrug-efflux transporters.
Mol Microbiol: 1999, 31(5);1549-59
[PubMed:10200972] [WorldCat.org] [DOI] (P p)

E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933] [WorldCat.org] [DOI] (P p)