Ffh

• Description: signal recognition particle (SRP) component
  
  

Gene name	ffh
Synonyms
Essential	yes PubMed
Product	signal recognition particle (SRP) component
Function	presecretory protein translocation
Interactions involving this protein in SubtInteract: Ffh
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	49 kDa, 9.422
Gene length, protein length	1338 bp, 446 aa
Immediate neighbours	ylxM, rpsP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

protein secretion, essential genes, membrane proteins

The gene

Basic information

• Locus tag: BSU15980

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: GTP-binding SRP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: SecA-Ffh PubMed, Ffh-Scr

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P37105

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ylxM-ffh PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Geeske Zanen, Haike Antelmann, Rob Meima, Jan D H Jongbloed, Marc Kolkman, Michael Hecker, Jan Maarten van Dijl, Wim J Quax
Proteomic dissection of potential signal recognition particle dependence in protein secretion by Bacillus subtilis.
Proteomics: 2006, 6(12);3636-48
[PubMed:16705751] [WorldCat.org] [DOI] (P p)

K Bunai, K Yamada, K Hayashi, K Nakamura, K Yamane
Enhancing effect of Bacillus subtilis Ffh, a homologue of the SRP54 subunit of the mammalian signal recognition particle, on the binding of SecA to precursors of secretory proteins in vitro.
J Biochem: 1999, 125(1);151-9
[PubMed:9880811] [WorldCat.org] [DOI] (P p)

H Takamatsu, K Bunai, T Horinaka, A Oguro, K Nakamura, K Watabe, K Yamane
Identification of a region required for binding to presecretory protein in Bacillus subtilis Ffh, a homologue of the 54-kDa subunit of mammalian signal recognition particle.
Eur J Biochem: 1997, 248(2);575-82
[PubMed:9346318] [WorldCat.org] [DOI] (P p)

K Honda, K Nakamura, M Nishiguchi, K Yamane
Cloning and characterization of a Bacillus subtilis gene encoding a homolog of the 54-kilodalton subunit of mammalian signal recognition particle and Escherichia coli Ffh.
J Bacteriol: 1993, 175(15);4885-94
[PubMed:8335643] [WorldCat.org] [DOI] (P p)