FliJ

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  • Description: flagellar protein required for formation of basal body

Gene name fliJ
Synonyms cheF
Essential no
Product flagellar protein required for formation of basal body
Function movement and chemotaxis
Interactions involving this protein in SubtInteract: FliJ
MW, pI 17 kDa, 8.409
Gene length, protein length 441 bp, 147 aa
Immediate neighbours fliI, ylxF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
FliJ context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16250

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: fliJ family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Gert Bange, Nico Kümmerer, Christoph Engel, Gunes Bozkurt, Klemens Wild, Irmgard Sinning
FlhA provides the adaptor for coordinated delivery of late flagella building blocks to the type III secretion system.
Proc Natl Acad Sci U S A: 2010, 107(25);11295-300
[PubMed:20534509] [WorldCat.org] [DOI] (I p)

Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253] [WorldCat.org] [DOI] (P p)

H Werhane, P Lopez, M Mendel, M Zimmer, G W Ordal, L M Márquez-Magaña
The last gene of the fla/che operon in Bacillus subtilis, ylxL, is required for maximal sigmaD function.
J Bacteriol: 2004, 186(12);4025-9
[PubMed:15175317] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

W Estacio, S S Anna-Arriola, M Adedipe, L M Márquez-Magaña
Dual promoters are responsible for transcription initiation of the fla/che operon in Bacillus subtilis.
J Bacteriol: 1998, 180(14);3548-55
[PubMed:9657996] [WorldCat.org] [DOI] (P p)

L M Márquez-Magaña, M J Chamberlin
Characterization of the sigD transcription unit of Bacillus subtilis.
J Bacteriol: 1994, 176(8);2427-34
[PubMed:8157612] [WorldCat.org] [DOI] (P p)

C W Ying, F Scoffone, A M Albertini, A Galizzi, G W Ordal
Properties of the Bacillus subtilis chemotaxis protein CheF, a homolog of the Salmonella typhimurium flagellar protein FliJ.
J Bacteriol: 1991, 173(11);3584-6
[PubMed:1904439] [WorldCat.org] [DOI] (P p)

C W Ying, G W Ordal
Nucleotide sequence and expression of cheF, an essential gene for chemotaxis in Bacillus subtilis.
J Bacteriol: 1989, 171(3);1631-7
[PubMed:2493450] [WorldCat.org] [DOI] (P p)