MtrB

• Description: tryptophan operon RNA-binding attenuation protein (TRAP), controls the RNA switch in front of genes involved in biosynthesis and acquisition of tryptophan
  
  

Gene name	mtrB
Synonyms
Essential	no
Product	tryptophan operon RNA-binding attenuation protein (TRAP)
Function	regulation of tryptophan biosynthesis (and translation) attenuation in the trp operon; repression of the folate operon
Interactions involving this protein in SubtInteract: TRAP
Metabolic function and regulation of this protein in SubtiPathways: Phe, Tyr, Trp, Folate
MW, pI	8 kDa, 7.333
Gene length, protein length	225 bp, 75 aa
Immediate neighbours	hepS, folE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, transcription factors and their control, RNA binding regulators

The gene

Basic information

• Locus tag: BSU22770

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: prpD family (according to Swiss-Prot)

• Paralogous protein(s):

Genes controlled by MtrB

• pabA PubMed
• trpE-trpD-trpC-trpF-trpB-trpA PubMed
• trpP PubMed
• ycbK PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • binding of tryptophan results RNA binding and thus in transcription termination

• Interactions:
  • forms 11-mers
  • TRAP-RtpA PubMed

• Localization:

Database entries

• Structure: 3AQD, 1WAP (complex with L-tryptophan), 2ZP9 (complex with RtpA)

• UniProt: P19466

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: folE-mtrB PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Paul Babitzke, Carol S Baker, Tony Romeo
Regulation of translation initiation by RNA binding proteins.
Annu Rev Microbiol: 2009, 63;27-44
[PubMed:19385727] [WorldCat.org] [DOI] (I p)

Paul Gollnick, Paul Babitzke, Alfred Antson, Charles Yanofsky
Complexity in regulation of tryptophan biosynthesis in Bacillus subtilis.
Annu Rev Genet: 2005, 39;47-68
[PubMed:16285852] [WorldCat.org] [DOI] (P p)