TilS

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  • Description: tRNAIle-lysidine synthetase

Gene name tilS
Synonyms yacA
Essential yes PubMed
Product tRNAIle-lysidine synthetase
Function tRNA modification
Metabolic function and regulation of this protein in SubtiPathways:
Nucleotides (regulation)
MW, pI 53 kDa, 8.317
Gene length, protein length 1416 bp, 472 aa
Immediate neighbours yabT, hprT
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YacA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TilS expression.png




























Categories containing this gene/protein

translation, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

  • Locus tag: BSU00670

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: tRNA(Ile)-lysidine synthase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3A2K (from Geobacillus kaustophilus, complex with tRNA) PubMed
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Tsutomu Suzuki, Kenjyo Miyauchi
Discovery and characterization of tRNAIle lysidine synthetase (TilS).
FEBS Lett: 2010, 584(2);272-7
[PubMed:19944692] [WorldCat.org] [DOI] (I p)

Henri Grosjean, Glenn R Björk
Enzymatic conversion of cytidine to lysidine in anticodon of bacterial isoleucyl-tRNA--an alternative way of RNA editing.
Trends Biochem Sci: 2004, 29(4);165-8
[PubMed:15124629] [WorldCat.org] [DOI] (P p)

Original publications