PolA

From SubtiWiki
Revision as of 14:48, 7 August 2012 by 134.76.70.252 (talk)
Jump to: navigation, search
  • Description: DNA polymerase I, required for repair of UV- or MMS-induced DNA damage

Gene name polA
Synonyms
Essential no
Product DNA polymerase I
Function DNA replication
Gene expression levels in SubtiExpress: polA
MW, pI 98 kDa, 4.916
Gene length, protein length 2640 bp, 880 aa
Immediate neighbours mutM, phoR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PolA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PolA expression.png
























Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU29090

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: has DNA polymerase and 5'-3' exonuclease activity, but no 3'-5' exonuclease activity PubMed
  • Protein family: DNA polymerase type-A family (according to Swiss-Prot)
  • Paralogous protein(s): YpcP, one of the two proteins has to be present for viability PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1XWL (large fragment, corresponds to AA 310 to 880, Geobacillus stearothermophilus)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Sanae Fukushima, Mitsuhiro Itaya, Hiroaki Kato, Naotake Ogasawara, Hirofumi Yoshikawa
Reassessment of the in vivo functions of DNA polymerase I and RNase H in bacterial cell growth.
J Bacteriol: 2007, 189(23);8575-83
[PubMed:17905985] [WorldCat.org] [DOI] (I p)

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)

Stéphane Duigou, S Dusko Ehrlich, Philippe Noirot, Marie-Françoise Noirot-Gros
DNA polymerase I acts in translesion synthesis mediated by the Y-polymerases in Bacillus subtilis.
Mol Microbiol: 2005, 57(3);678-90
[PubMed:16045613] [WorldCat.org] [DOI] (P p)