GltX

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Gene name gltX
Synonyms
Essential yes PubMed
Product glutamyl-tRNA synthetase
Function translation
Gene expression levels in SubtiExpress: gltX
Interactions involving this protein in SubtInteract: GltX
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging, Cys, Met & Sulfate assimilation
MW, pI 55 kDa, 5.021
Gene length, protein length 1449 bp, 483 aa
Immediate neighbours ispF, cysE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GltX context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GltX expression.png























Categories containing this gene/protein

translation, essential genes, universally conserved proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU00920

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu) (according to Swiss-Prot)
  • Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3AKZ (complex with tRNA-Gln from Thermotoga maritima, 38% identity, 62% similarity) PubMed
  • KEGG entry: [3]

Additional information

universally conserved protein

Expression and regulation

  • Regulation:
    • expression transiently increases in the forespore PubMed
  • Regulatory mechanism:
  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Alex Rosenberg, Lior Sinai, Yoav Smith, Sigal Ben-Yehuda
Dynamic expression of the translational machinery during Bacillus subtilis life cycle at a single cell level.
PLoS One: 2012, 7(7);e41921
[PubMed:22848659] [WorldCat.org] [DOI] (I p)

Takuhiro Ito, Shigeyuki Yokoyama
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Nature: 2010, 467(7315);612-6
[PubMed:20882017] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)