GlpD

From SubtiWiki
Revision as of 11:06, 10 August 2012 by Raphael2215 (talk | contribs)
Jump to: navigation, search
  • Description: glycerol-3-phosphate dehydrogenase (menaquinone 7)

Gene name glpD
Synonyms
Essential no
Product glycerol-3-phosphate dehydrogenase (menaquinone 7)
Function glycerol utilization
Gene expression levels in SubtiExpress: glpD
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 62 kDa, 8.002
Gene length, protein length 1665 bp, 555 aa
Immediate neighbours glpK, pgcA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GlpD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GlpD expression.png




























Categories containing this gene/protein

utilization of specific carbon sources, utilization of lipids

This gene is a member of the following regulons

AbrB regulon, CcpA regulon, GlpP regulon

The gene

Basic information

  • Locus tag: BSU09300

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol (according to Swiss-Prot)
  • Protein family: FAD-dependent glycerol-3-phosphate dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): FAD
  • Effectors of protein activity:

Database entries

  • Structure: 3DA1 (from Bacillus halodurans, 57% identity, 72% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant: QB5347 (cat), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

E Glatz, A Farewell, B Rutberg
The Bacillus subtilis glpD leader and antiterminator protein GlpP provide a target for glucose repression in Escherichia coli.
FEMS Microbiol Lett: 1998, 162(1);93-6
[PubMed:9595668] [WorldCat.org] [DOI] (P p)

Elisabeth Glatz, Martin Persson, Blanka Rutberg
Antiterminator protein GlpP of Bacillus subtilis binds to glpD leader mRNA.
Microbiology (Reading): 1998, 144 ( Pt 2);449-456
[PubMed:9493382] [WorldCat.org] [DOI] (P p)

E Glatz, R P Nilsson, L Rutberg, B Rutberg
A dual role for the Bacillus subtilis glpD leader and the GlpP protein in the regulated expression of glpD: antitermination and control of mRNA stability.
Mol Microbiol: 1996, 19(2);319-28
[PubMed:8825777] [WorldCat.org] [DOI] (P p)

C Holmberg, L Rutberg
An inverted repeat preceding the Bacillus subtilis glpD gene is a conditional terminator of transcription.
Mol Microbiol: 1992, 6(20);2931-8
[PubMed:1479885] [WorldCat.org] [DOI] (P p)