CcpC

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  • Description: transcriptional repressor of citZ and citB

Gene name ccpC
Synonyms ykuM
Essential no
Product transcriptional repressor (LysR family)
Function regulation of tricarboxylic acid branch of the TCA cycle
Gene expression levels in SubtiExpress: ccpC
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 33 kDa, 5.932
Gene length, protein length 879 bp, 293 aa
Immediate neighbours ykuL, ykuN
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CcpC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CcpC expression.png




























Categories containing this gene/protein

carbon core metabolism, transcription factors and their control, regulators of core metabolism

This gene is a member of the following regulons

CcpA regulon, CcpC regulon

The CcpC regulon:

The gene

Basic information

  • Locus tag: BSU14140

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed in the presence of glucose and glutamate (CcpC) PubMed
  • Regulatory mechanism:
    • CcpC: transcription repression (molecular inducer: citrate) PubMed
    • CcpA: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant: GP206 (aphA3) & GP1442 (aphA3) , available in Stülke lab
  • Expression vector:
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP706, available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Your additional remarks

References