DefB

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  • Description: major formylmethionine deformylase

Gene name ykrB
Synonyms defB
Essential no
Product formylmethionine deformylase
Function translation
Gene expression levels in SubtiExpress: ykrB
MW, pI 20 kDa, 5.456
Gene length, protein length 552 bp, 184 aa
Immediate neighbours ykrA, ykzV
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YkrB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkrB expression.png















Categories containing this gene/protein

translation, protein modification

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU14560

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Formyl-L-methionyl peptide + H2O = formate + methionyl peptide (according to Swiss-Prot)
  • Protein family: polypeptide deformylase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1LQY (complex with antibiotic actinonin, Geobacillus stearothermophilus)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Michael Haas, Dieter Beyer, Reinhold Gahlmann, Christoph Freiberg
YkrB is the main peptide deformylase in Bacillus subtilis, a eubacterium containing two functional peptide deformylases.
Microbiology (Reading): 2001, 147(Pt 7);1783-1791
[PubMed:11429456] [WorldCat.org] [DOI] (P p)