YaaH

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  • Description: general stress protein, survival of ethanol stress, SafA-dependent spore coat protein, spore cortex lytic protein

Gene name yaaH
Synonyms sleL
Essential no
Product N-acetylglucosaminidase
Function survival of ethanol stress, protection of the spore
Gene expression levels in SubtiExpress: yaaH
MW, pI 48 kDa, 5.618
Gene length, protein length 1281 bp, 427 aa
Immediate neighbours dgk, yaaI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YaaH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YaaH expression.png















Categories containing this gene/protein

sporulation proteins, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

SigB regulon, SigE regulon, SpoIIID regulon

The gene

Basic information

  • Locus tag: BSU00160

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

required for survival of ethanol stress PubMed

Expression and regulation

  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Emily A Lambert, Nora Sherry, David L Popham
In vitro and in vivo analyses of the Bacillus anthracis spore cortex lytic protein SleL.
Microbiology (Reading): 2012, 158(Pt 5);1359-1368
[PubMed:22343356] [WorldCat.org] [DOI] (I p)

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Daisuke Imamura, Ritsuko Kuwana, Hiromu Takamatsu, Kazuhito Watabe
Localization of proteins to different layers and regions of Bacillus subtilis spore coats.
J Bacteriol: 2010, 192(2);518-24
[PubMed:19933362] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Takenori Satomura, Yasutaro Fujita, Kozo Ochi
Novel gene regulation mediated by overproduction of secondary metabolite neotrehalosadiamine in Bacillus subtilis.
FEMS Microbiol Lett: 2009, 291(2);151-6
[PubMed:19087206] [WorldCat.org] [DOI] (I p)

Emily A Lambert, David L Popham
The Bacillus anthracis SleL (YaaH) protein is an N-acetylglucosaminidase involved in spore cortex depolymerization.
J Bacteriol: 2008, 190(23);7601-7
[PubMed:18835992] [WorldCat.org] [DOI] (I p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

A Aronson
Sporulation and delta-endotoxin synthesis by Bacillus thuringiensis.
Cell Mol Life Sci: 2002, 59(3);417-25
[PubMed:11964120] [WorldCat.org] [DOI] (P p)

T Kodama, H Takamatsu, K Asai, K Kobayashi, N Ogasawara, K Watabe
The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.
J Bacteriol: 1999, 181(15);4584-91
[PubMed:10419957] [WorldCat.org] [DOI] (P p)