MtlR

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  • Description: transcriptional activator of the mtlA-mtlF-mtlD operon

Gene name mtlR
Synonyms ydaA
Essential no
Product transcriptional activator, PRD-type
Function regulation of mannitol utilization
Gene expression levels in SubtiExpress: mtlR
Interactions involving this protein in SubtInteract: MtlR
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 78 kDa, 5.313
Gene length, protein length 2082 bp, 694 aa
Immediate neighbours ycsN, ydaB
Sequences Protein DNA Advanced_DNA
Genetic context
MtlR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MtlR expression.png















Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

CcpA regulon

The MtlR regulon:

The gene

Basic information

  • Locus tag: BSU04160

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: N-terminal DNA-binding domains, two PTS-regulation domains (PRD1 and PRD2), EIIB (Gat)-like domain, EIIA (Mtl)-like domain
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • activity is stimulated by PtsH-dependent phosphorylation in PRD2 (mechnism of carbon catabolite repression) PubMed
    • activity is inhibited by MtlF-dependent phosphorylation in the EIIB(Gat)-like domain on Cys-419 (this prevents activity in the absence of mannitol and allows induction in presence of mannitol) PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Philippe Joyet, Houda Bouraoui, Francine Moussan Désirée Aké, Meriem Derkaoui, Arthur Constant Zébré, Thanh Nguyen Cao, Magali Ventroux, Sylvie Nessler, Marie-Françoise Noirot-Gros, Josef Deutscher, Eliane Milohanic
Transcription regulators controlled by interaction with enzyme IIB components of the phosphoenolpyruvate: sugar phosphotransferase system.
Biochim Biophys Acta: 2013, 1834(7);1415-24
[PubMed:23318733] [WorldCat.org] [DOI] (P p)

J Stülke, M Arnaud, G Rapoport, I Martin-Verstraete
PRD--a protein domain involved in PTS-dependent induction and carbon catabolite repression of catabolic operons in bacteria.
Mol Microbiol: 1998, 28(5);865-74
[PubMed:9663674] [WorldCat.org] [DOI] (P p)


Original publications

Additional publications: PubMed