TrpS

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  • Description: tryptophanyl-tRNA synthetase

Gene name trpS
Synonyms
Essential yes PubMed
Product tryptophanyl-tRNA synthetase
Function translation
Gene expression levels in SubtiExpress: trpS
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 37 kDa, 6.542
Gene length, protein length 990 bp, 330 aa
Immediate neighbours yjbA, oppA
Sequences Protein DNA Advanced_DNA
Genetic context
TrpS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TrpS expression.png




























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU11420

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp) (according to Swiss-Prot)
  • Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
    • 3PRH (Val144Pro mutant) PubMed
    • 1BM2 (complex with tryptophan, Geobacillus stearothermophilus)
    • 1MAW (complex with ATP, Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
    • T-box: transcriptional antitermination in the absence of tryptophan (upon tRNA-binding to the mRNA leader region) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Alicja K Antonczak, Zuzana Simova, Isaac T Yonemoto, Matthias Bochtler, Anna Piasecka, Honorata Czapinska, Andrea Brancale, Eric M Tippmann
Importance of single molecular determinants in the fidelity of expanded genetic codes.
Proc Natl Acad Sci U S A: 2011, 108(4);1320-5
[PubMed:21224416] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

A I Lee, J P Sarsero, C Yanofsky
A temperature-sensitive trpS mutation interferes with trp RNA-binding attenuation protein (TRAP) regulation of trp gene expression in Bacillus subtilis.
J Bacteriol: 1996, 178(22);6518-24
[PubMed:8932308] [WorldCat.org] [DOI] (P p)

C Condon, M Grunberg-Manago, H Putzer
Aminoacyl-tRNA synthetase gene regulation in Bacillus subtilis.
Biochimie: 1996, 78(6);381-9
[PubMed:8915527] [WorldCat.org] [DOI] (P p)