XylB

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  • Description: xylulokinase

Gene name xylB
Synonyms yncA
Essential no
Product xylulokinase
Function utilization of xylan and xylose
Gene expression levels in SubtiExpress: xylB
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 55 kDa, 6.459
Gene length, protein length 1497 bp, 499 aa
Immediate neighbours xylA, yncB
Sequences Protein DNA Advanced_DNA
Genetic context
XylB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
XylB expression.png




























Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, XylR regulon

The gene

Basic information

  • Locus tag: BSU17610

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + D-xylulose = ADP + D-xylulose 5-phosphate (according to Swiss-Prot)
  • Protein family: FGGY kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2NLX (from E. coli, 35% identity, 52% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant: GP1151 (del aphA3) available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Wolfgang Hillen, Erlangen University, Germany Homepage

Your additional remarks

References

Eric Di Luccio, Barbara Petschacher, Jennifer Voegtli, Hui-Ting Chou, Henning Stahlberg, Bernd Nidetzky, David K Wilson
Structural and kinetic studies of induced fit in xylulose kinase from Escherichia coli.
J Mol Biol: 2007, 365(3);783-98
[PubMed:17123542] [WorldCat.org] [DOI] (P p)

A Kraus, C Hueck, D Gärtner, W Hillen
Catabolite repression of the Bacillus subtilis xyl operon involves a cis element functional in the context of an unrelated sequence, and glucose exerts additional xylR-dependent repression.
J Bacteriol: 1994, 176(6);1738-45
[PubMed:8132469] [WorldCat.org] [DOI] (P p)

S Jacob, R Allmansberger, D Gärtner, W Hillen
Catabolite repression of the operon for xylose utilization from Bacillus subtilis W23 is mediated at the level of transcription and depends on a cis site in the xylA reading frame.
Mol Gen Genet: 1991, 229(2);189-96
[PubMed:1921970] [WorldCat.org] [DOI] (P p)

D Gärtner, M Geissendörfer, W Hillen
Expression of the Bacillus subtilis xyl operon is repressed at the level of transcription and is induced by xylose.
J Bacteriol: 1988, 170(7);3102-9
[PubMed:2454911] [WorldCat.org] [DOI] (P p)