CsaA

From SubtiWiki
Revision as of 10:24, 14 May 2013 by 134.76.70.252 (talk)
Jump to: navigation, search
  • Description: molecular chaperone involved in protein secretion

Gene name csaA
Synonyms
Essential no
Product molecular chaperone
Function protein secretion
Gene expression levels in SubtiExpress: csaA
Interactions involving this protein in SubtInteract: CsaA
Metabolic function and regulation of this protein in SubtiPathways:
Protein secretion
MW, pI 11 kDa, 8.691
Gene length, protein length 330 bp, 110 aa
Immediate neighbours bsrF, yobQ
Sequences Protein DNA DNA_with_flanks
Genetic context
CsaA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CsaA expression.png
























Categories containing this gene/protein

protein secretion, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU19040

Phenotypes of a mutant

From unsuccessful trials to knock out the gene it was concluded that csaA apparently is essential. However, in a conditional mutant (Pspac-csaA), depletion of the gene product is not lethal. But secretion of some proteins including SdpC was reported to be affected unter such conditions.PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Secretion associated chaperone. Assumed functional analogue of E. coli SecB
  • Protein family: FIG1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: CsaA contains a tRNA binding domain.
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

The functional entity of CsaA is the homodimer.

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Yuliya A Shapova, Mark Paetzel
Crystallographic analysis of Bacillus subtilis CsaA.
Acta Crystallogr D Biol Crystallogr: 2007, 63(Pt 4);478-85
[PubMed:17372352] [WorldCat.org] [DOI] (P p)

Dirk Linde, Rudolf Volkmer-Engert, Sandra Schreiber, Jörg P Müller
Interaction of the Bacillus subtilis chaperone CsaA with the secretory protein YvaY.
FEMS Microbiol Lett: 2003, 226(1);93-100
[PubMed:13129613] [WorldCat.org] [DOI] (P p)

J P Müller, J Ozegowski, S Vettermann, J Swaving, K H Van Wely, A J Driessen
Interaction of Bacillus subtilis CsaA with SecA and precursor proteins.
Biochem J: 2000, 348 Pt 2(Pt 2);367-73
[PubMed:10816431] [WorldCat.org] (P p)

Jörg P Müller, Sierd Bron, Gerard Venema, Jan Maarten van Dijl
Chaperone-like activities of the CsaA protein of Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 1);77-88
[PubMed:10658654] [WorldCat.org] [DOI] (P p)