GltX

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Gene name gltX
Synonyms
Essential yes PubMed
Product glutamyl-tRNA synthetase
Function translation
Gene expression levels in SubtiExpress: gltX
Interactions involving this protein in SubtInteract: GltX
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging, Cys, Met & Sulfate assimilation
MW, pI 55 kDa, 5.021
Gene length, protein length 1449 bp, 483 aa
Immediate neighbours ispF, cysE
Sequences Protein DNA DNA_with_flanks
Genetic context
GltX context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GltX expression.png























Categories containing this gene/protein

translation, essential genes, universally conserved proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU00920

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu) (according to Swiss-Prot)
  • Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3AKZ (complex with tRNA-Gln from Thermotoga maritima, 38% identity, 62% similarity) PubMed
  • KEGG entry: [3]

Additional information

universally conserved protein

Expression and regulation

  • Regulation:
    • expression transiently increases in the forespore PubMed
  • Regulatory mechanism:
  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Alex Rosenberg, Lior Sinai, Yoav Smith, Sigal Ben-Yehuda
Dynamic expression of the translational machinery during Bacillus subtilis life cycle at a single cell level.
PLoS One: 2012, 7(7);e41921
[PubMed:22848659] [WorldCat.org] [DOI] (I p)

Takuhiro Ito, Shigeyuki Yokoyama
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Nature: 2010, 467(7315);612-6
[PubMed:20882017] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)