StoA

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  • Description: thiol-disulfide oxidoreductase, with thioredoxin-like domain, required for the synthesis of the endospore peptidoglycan cortex

Gene name stoA
Synonyms spoIVH, ykvV
Essential no
Product thiol-disulfide oxidoreductase
Function spore cortex formation
Gene expression levels in SubtiExpress: stoA
Interactions involving this protein in SubtInteract: StoA
MW, pI 18 kDa, 5.297
Gene length, protein length 495 bp, 165 aa
Immediate neighbours ykvU, zosA
Sequences Protein DNA DNA_with_flanks
Genetic context
YkvV context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
StoA expression.png
























Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigE regulon, SigG regulon

The gene

Basic information

  • Locus tag: BSU13840

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: reduction of intramolecular disulfide bonds in SpoVD PubMed
  • Protein family: thioredoxin domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Lars Hederstedt, University of Lund, Sweden Homepage

Your additional remarks

References

Reviews

Patrick Eichenberger
The red-ox status of a penicillin-binding protein is an on/off switch for spore peptidoglycan synthesis in Bacillus subtilis.
Mol Microbiol: 2010, 75(1);10-2
[PubMed:19919674] [WorldCat.org] [DOI] (I p)

Original Publications

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Yiming Liu, Mirja Carlsson Möller, Lise Petersen, Christopher A G Söderberg, Lars Hederstedt
Penicillin-binding protein SpoVD disulphide is a target for StoA in Bacillus subtilis forespores.
Mol Microbiol: 2010, 75(1);46-60
[PubMed:19919673] [WorldCat.org] [DOI] (I p)

Allister Crow, Yiming Liu, Mirja Carlsson Möller, Nick E Le Brun, Lars Hederstedt
Structure and functional properties of Bacillus subtilis endospore biogenesis factor StoA.
J Biol Chem: 2009, 284(15);10056-66
[PubMed:19144642] [WorldCat.org] [DOI] (P p)

Lyethur S Erlendsson, Mirja Möller, Lars Hederstedt
Bacillus subtilis StoA Is a thiol-disulfide oxidoreductase important for spore cortex synthesis.
J Bacteriol: 2004, 186(18);6230-8
[PubMed:15342593] [WorldCat.org] [DOI] (P p)

Daisuke Imamura, Kazuo Kobayashi, Junichi Sekiguchi, Naotake Ogasawara, Michio Takeuchi, Tsutomu Sato
spoIVH (ykvV), a requisite cortex formation gene, is expressed in both sporulating compartments of Bacillus subtilis.
J Bacteriol: 2004, 186(16);5450-9
[PubMed:15292147] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)