Tgl

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  • Description: spore associated transglutaminase

Gene name tgl
Synonyms yuxF, yugV
Essential no
Product spore associated transglutaminase
Function introduction of crosslinks in the spore coat protein GerQ
Gene expression levels in SubtiExpress: tgl
MW, pI 28 kDa, 6.687
Gene length, protein length 735 bp, 245 aa
Immediate neighbours mcpB, yuzH
Sequences Protein DNA DNA_with_flanks
Genetic context
Tgl context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Tgl expression.png
























Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

  • Locus tag: BSU31270

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3 (according to Swiss-Prot), temperature-dependent modification of the coat proteins such as GerQ (together with YabG) PubMed
  • Protein family: bacillus TGase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the mother cell (SigK) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Adriano Henriques, Lisbon, Portugal homepage

Your additional remarks

References

Reviews

Ritsuko Kuwana
[Protein modification system in dormant cells].
Yakugaku Zasshi: 2009, 129(10);1221-5
[PubMed:19797877] [WorldCat.org] [DOI] (P p)

Original Publications

Diana Plácido, Catarina G Fernandes, Anabela Isidro, Maria Arménia Carrondo, Adriano O Henriques, Margarida Archer
Auto-induction and purification of a Bacillus subtilis transglutaminase (Tgl) and its preliminary crystallographic characterization.
Protein Expr Purif: 2008, 59(1);1-8
[PubMed:18249137] [WorldCat.org] [DOI] (I p)

Ritsuko Kuwana, Naoyuki Okuda, Hiromu Takamatsu, Kazuhito Watabe
Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores.
J Biochem: 2006, 139(5);887-901
[PubMed:16751597] [WorldCat.org] [DOI] (P p)

Rita Zilhão, Rachele Isticato, Lígia O Martins, Leif Steil, Uwe Völker, Ezio Ricca, Charles P Moran, Adriano O Henriques
Assembly and function of a spore coat-associated transglutaminase of Bacillus subtilis.
J Bacteriol: 2005, 187(22);7753-64
[PubMed:16267299] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Katsunori Kobayashi, Shun-Ichi Suzuki, Yuko Izawa, Kiyoshi Miwa, Shigeru Yamanaka
Transglutaminase in sporulating cells of Bacillus subtilis.
J Gen Appl Microbiol: 1998, 44(1);85-91
[PubMed:12501297] [WorldCat.org] [DOI] (I p)

S Suzuki, Y Izawa, K Kobayashi, Y Eto, S Yamanaka, K Kubota, K Yokozeki
Purification and characterization of novel transglutaminase from Bacillus subtilis spores.
Biosci Biotechnol Biochem: 2000, 64(11);2344-51
[PubMed:11193401] [WorldCat.org] [DOI] (P p)

Bauke Oudega, Gregory Koningstein, Luísa Rodrigues, Maria de Sales Ramon, Helmut Hilbert, Andreas Düsterhöft, Thomas M Pohl, Thomas Weitzenegger
Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).
Microbiology (Reading): 1997, 143 ( Pt 8);2769-2774
[PubMed:9274030] [WorldCat.org] [DOI] (P p)