RocA

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  • Description: 3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase

Gene name rocA
Synonyms ipa-76d
Essential no
Product 3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase
Function arginine, ornithine and citrulline utilization
Gene expression levels in SubtiExpress: rocA
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 56 kDa, 5.58
Gene length, protein length 1545 bp, 515 aa
Immediate neighbours rocB, rocG
Sequences Protein DNA DNA_with_flanks
Genetic context
RocA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RocA expression.png















Categories containing this gene/protein

utilization of amino acids, phosphoproteins

This gene is a member of the following regulons

AbrB regulon, AhrC regulon, CodY regulon, RocR regulon, SigL regulon

The gene

Basic information

  • Locus tag: BSU37780

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H (according to Swiss-Prot)
  • Protein family: RocA subfamily (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on (Thr-2 OR Thr-4 OR Tyr-5) PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3RJL (1-pyrroline-5-carboxylate dehydrogenase from Bacillus licheniformis, 68% identity, 88% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induction by arginine (RocR, AhrC) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References