LytE

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  • Description: cell wall hydrolase (major autolysin) for cell elongation and separation, D,L-endopeptidase-type autolysin

Gene name lytE
Synonyms papQ, cwlF
Essential no
Product cell wall hydrolase (major autolysin),endopeptidase-type autolysin
Function major autolysin, cell elongation and separation
Gene expression levels in SubtiExpress: lytE
MW, pI 37 kDa, 10.713
Gene length, protein length 1029 bp, 343 aa
Immediate neighbours phoA, citR
Sequences Protein DNA DNA_with_flanks
Genetic context
LytE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LytE expression.png















Categories containing this gene/protein

cell wall degradation/ turnover, cell wall synthesis


This gene is a member of the following regulons

SigH regulon, SigI regulon, Spo0A regulon, WalR regulon

The gene

Basic information

  • Locus tag: BSU09420

Phenotypes of a mutant

  • a cwlO lytE mutant is not viable PubMed
  • growth defect at high temperature PubMed
  • inactivation of lytE strongly restores beta-lactam resistance in a sigM mutant by delaying cell lysis PubMed
  • a lytE mutation is synthetically lethal with ftsE and ftsX mutation (due to a lack of autolysin activity) PubMed
  • a lytE mutation increases the cell separation defect of a lytF mutant PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: nlpC/p60 family (according to Swiss-Prot)
  • Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity

Extended information on the protein

  • Kinetic information:
  • Domains:
    • C-terminal D,L-endopeptidase domain PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • secreted (according to Swiss-Prot)
    • binds the cell wall PubMed
    • localizes to cell septa, poles and lateral sidewall of the cell (via the N-terminal domain) PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
    • induced at high temperature (SigI) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Waldemar Vollmer
Bacterial growth does require peptidoglycan hydrolases.
Mol Microbiol: 2012, 86(5);1031-5
[PubMed:23066944] [WorldCat.org] [DOI] (I p)

Original publications