Mfd

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  • Description: transcription-repair coupling factor, eliminates genetic damage from transcriptionally active genes during sporulation

Gene name mfd
Synonyms
Essential no
Product transcription-repair coupling factor
Function promotes strand-specific DNA repair by displacing

RNA polymerase stalled at a nucleotide lesion and directing

the (A)BC excinuclease to the RNA damage site

Gene expression levels in SubtiExpress: mfd

RNA polymerase stalled at a nucleotide lesion and directing

the (A)BC excinuclease to the RNA damage site

Interactions involving this protein in SubtInteract: Mfd
MW, pI 133 kDa, 5.367
Gene length, protein length 3531 bp, 1177 aa
Immediate neighbours fin, spoVT
Sequences Protein DNA DNA_with_flanks
Genetic context
Mfd context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Mfd expression.png















Categories containing this gene/protein

DNA repair/ recombination, transcription

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU00550

Phenotypes of a mutant

  • in an mfd knock-out, the cell's ability to accumulate adaptive mutations in stationary phase is depressed. PubMed
  • increased UV-induced mutagenesis via PolY1/ PolY2-mediated translesion synthesis PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • promotes strand-specific DNA repair by displacing RNA polymerase stalled at a nucleotide lesion and directing the (A)BC excinuclease to the RNA damage site
    • is required for roadblock transcription repression by transcription factors with binding sites downstream of the promoter (as for CcpA PubMed and CodY PubMed)
  • Protein family:
  • Paralogous protein(s): RecG

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
    • 2EYQ (Mfd from E. coli) PubMed
    • 3MLQ (RNA polymerase interacting domain of Thermus thermophilus Mfd with the Thermus aquaticus RpoB beta1 domain) PubMed
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1167 (del ermC), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • two-hybrid system:
  • Antibody:
  • Strep-tag construct: GP890 (spc, based on pGP1389), available in the Stülke lab

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications