GlcK

• Description: glucose kinase (D-glucose:ATP)
  
  

Gene name	glcK
Synonyms	yqgR
Essential	no
Product	glucose kinase (D-glucose:ATP)
Function	phosphorylation of the free glucose moiety resulting from cleavage of di-and oligosaccharides
Gene expression levels in SubtiExpress: glcK
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism
MW, pI	33 kDa, 5.988
Gene length, protein length	963 bp, 321 aa
Immediate neighbours	yqgS, yqgQ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, membrane proteins

The gene

Basic information

• Locus tag: BSU24850

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-glucose = ADP + D-glucose 6-phosphate (according to Swiss-Prot)

• Protein family: ROK (nagC/xylR) family (according to Swiss-Prot)

• Paralogous protein(s): XylR

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure: 2QM1 (from Enterococcus faecalis, 38% identity, 52% similarity)

• UniProt: P54495

• KEGG entry: [2]

• E.C. number: 2.7.1.2

Expression and regulation

• Operon:

• Expression browser: glcK PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP2 (spc), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Stefan Schönert, Sabine Seitz, Holger Krafft, Eva-Anne Feuerbaum, Iris Andernach, Gabriele Witz, Michael K Dahl
Maltose and maltodextrin utilization by Bacillus subtilis.
J Bacteriol: 2006, 188(11);3911-22
[PubMed:16707683] [WorldCat.org] [DOI] (P p)

Lili R Mesak, Felix M Mesak, Michael K Dahl
Expression of a novel gene, gluP, is essential for normal Bacillus subtilis cell division and contributes to glucose export.
BMC Microbiol: 2004, 4;13
[PubMed:15050034] [WorldCat.org] [DOI] (I e)

Lili R Mesak, Felix M Mesak, Michael K Dahl
Bacillus subtilis GlcK activity requires cysteines within a motif that discriminates microbial glucokinases into two lineages.
BMC Microbiol: 2004, 4;6
[PubMed:15018644] [WorldCat.org] [DOI] (I e)

P Skarlatos, M K Dahl
The glucose kinase of Bacillus subtilis.
J Bacteriol: 1998, 180(12);3222-6
[PubMed:9620975] [WorldCat.org] [DOI] (P p)

F Titgemeyer, J Reizer, A Reizer, M H Saier
Evolutionary relationships between sugar kinases and transcriptional repressors in bacteria.
Microbiology (Reading): 1994, 140 ( Pt 9);2349-54
[PubMed:7952186] [WorldCat.org] [DOI] (P p)