DctP

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  • Description: uptake of succinate, fumurate, malate and oxaloacetate via proton symport

Gene name dctP
Synonyms ydbH, dctA
Essential no
Product C4-dicarboxylate transport protein
Function uptake of succinate, fumurate, malate and oxaloacetate
Gene expression levels in SubtiExpress: dctP
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 45 kDa, 8.668
Gene length, protein length 1263 bp, 421 aa
Immediate neighbours dctR, ydbI
Sequences Protein DNA DNA_with_flanks
Genetic context
DctP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DctP expression.png















Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

CcpA regulon, FsrA regulon

The gene

Basic information

  • Locus tag: BSU04470

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: uptake of succinate, fumurate, malate and oxaloacetate via proton symport PubMed
  • Protein family: View classification (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 4KY0 (the glutamate transporter of Thermococcus kodakarensis, 31% identity, 71% similarity) PubMed
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed by glucose (6.2-fold) (CcpA) PubMed
    • part of the iron sparing response, strong down-regulation in a fur mutant (Fur, FsrA) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Sonja Jensen, Albert Guskov, Stephan Rempel, Inga Hänelt, Dirk Jan Slotboom
Crystal structure of a substrate-free aspartate transporter.
Nat Struct Mol Biol: 2013, 20(10);1224-6
[PubMed:24013209] [WorldCat.org] [DOI] (I p)

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

Gregory T Smaldone, Olga Revelles, Ahmed Gaballa, Uwe Sauer, Haike Antelmann, John D Helmann
A global investigation of the Bacillus subtilis iron-sparing response identifies major changes in metabolism.
J Bacteriol: 2012, 194(10);2594-605
[PubMed:22389480] [WorldCat.org] [DOI] (I p)

Maarten Groeneveld, Ruud G J Detert Oude Weme, Ria H Duurkens, Dirk Jan Slotboom
Biochemical characterization of the C4-dicarboxylate transporter DctA from Bacillus subtilis.
J Bacteriol: 2010, 192(11);2900-7
[PubMed:20363944] [WorldCat.org] [DOI] (I p)

Kousei Tanaka, Kazuo Kobayashi, Naotake Ogasawara
The Bacillus subtilis YufLM two-component system regulates the expression of the malate transporters MaeN (YufR) and YflS, and is essential for utilization of malate in minimal medium.
Microbiology (Reading): 2003, 149(Pt 9);2317-2329
[PubMed:12949159] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

Kei Asai, Sang-Hoon Baik, Yasuhiro Kasahara, Shigeki Moriya, Naotake Ogasawara
Regulation of the transport system for C4-dicarboxylic acids in Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 2);263-271
[PubMed:10708364] [WorldCat.org] [DOI] (P p)

Ralf Rabus, Donald L Jack, David J Kelly, Milton H Saier
TRAP transporters: an ancient family of extracytoplasmic solute-receptor-dependent secondary active transporters.
Microbiology (Reading): 1999, 145 ( Pt 12);3431-3445
[PubMed:10627041] [WorldCat.org] [DOI] (P p)