MutL

• Description: DNA mismatch repair
  
  

Gene name	mutL
Synonyms
Essential	no
Product	DNA mismatch repair
Function	DNA repair
Gene expression levels in SubtiExpress: mutL
Interactions involving this protein in SubtInteract: MutL
MW, pI	70 kDa, 5.583
Gene length, protein length	1881 bp, 627 aa
Immediate neighbours	mutS, ymzD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU17050

Phenotypes of a mutant

• 60-fold increased mutation rate PubMed
• a rnhB mutS-mutL mutant is not viable PubMed

Database entries

• BsubCyc: BSU17050

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: DNA mismatch repair mutL/hexB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • MutL (C-terminal domain)-DnaN PubMed
  • DnaE-MutL PubMed
  • MutL-MutS PubMed

• Localization:
  • forms foci at midcell position, the frequency of foci increases upon mismatch formation PubMed

Database entries

• BsubCyc: BSU17050

• Structure: 3KDK (C-terminal domain) PubMed

• UniProt: P49850

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mutS-mutL PubMed

• Expression browser: mutL PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • A ncRNA is predicted between mutL and ymzD PubMed

Biological materials

• Mutant:
  • GP1190 (del mutS-mutL::aphA3) PubMed, available in Jörg Stülke's lab
  • 1A833 ( mutL::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)