FadR

• Description: transcriptional repressor (TetR family), regulation of fatty acid degradation
  
  

Gene name	fadR
Synonyms	ysiA
Essential	no
Product	transcriptional repressor
Function	regulation of fatty acid degradation
Gene expression levels in SubtiExpress: fadR
Metabolic function and regulation of this protein in SubtiPathways: fadR
MW, pI	21 kDa, 6.118
Gene length, protein length	582 bp, 194 aa
Immediate neighbours	fadB, lcfA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of lipids, transcription factors and their control

This gene is a member of the following regulons

CcpA regulon, FadR regulon

The gene

Basic information

• Locus tag: BSU28550

Database entries

• BsubCyc: BSU28550

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: TetR family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity: long chain fatty acids act as inducers that release FadR from its operator sites PubMed

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU28550

• Structure:

• UniProt: P94548

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • lcfA-fadR-fadB-etfB-etfA PubMed
  • fadR-fadB-etfB-etfA PubMed

• Expression browser: fadR PubMed

• Sigma factor:
  • lcfA: SigA PubMed
  • fadR: SigA PubMed

• Regulation:
  • induced by long-chain fatty acids (FadR) PubMed
  • subject to carbon catabolite repression (CcpA-HPr(Ser-P) PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed
  • CcpA-HPr(Ser-P): transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A853 ( fadR::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Leslie Cuthbertson, Justin R Nodwell
The TetR family of regulators.
Microbiol Mol Biol Rev: 2013, 77(3);440-75
[PubMed:24006471] [WorldCat.org] [DOI] (I p)

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Original publications

Masahiro Fujihashi, Taiga Nakatani, Kazutake Hirooka, Hiroshi Matsuoka, Yasutaro Fujita, Kunio Miki
Structural characterization of a ligand-bound form of Bacillus subtilis FadR involved in the regulation of fatty acid degradation.
Proteins: 2014, 82(7);1301-10
[PubMed:24356978] [WorldCat.org] [DOI] (I p)

Shigeo Tojo, Takenori Satomura, Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Catabolite repression of the Bacillus subtilis FadR regulon, which is involved in fatty acid catabolism.
J Bacteriol: 2011, 193(10);2388-95
[PubMed:21398533] [WorldCat.org] [DOI] (I p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)