Pta

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  • Description: phosphotransacetylase

Gene name pta
Synonyms ipa-88d, ywfJ
Essential no
Product phosphotransacetylase
Function overflow metabolism
Gene expression levels in SubtiExpress: pta
Metabolic function and regulation of this protein in SubtiPathways:
pta
MW, pI 34 kDa, 4.649
Gene length, protein length 969 bp, 323 aa
Immediate neighbours cysL, ywfI
Sequences Protein DNA DNA_with_flanks
Genetic context
Pta context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Pta expression.png
















Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

  • Locus tag: BSU37660

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Acetyl-CoA + phosphate = CoA + acetyl phosphate (according to Swiss-Prot)
  • Protein family: phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on (Ser-123 OR Thr-128 OR Ser-129) PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1XCO (complex with acetylphosphate), 1TD9
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expression activated by glucose (2.6 fold) (CcpA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP668 (aphA3), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Alan J Wolfe
The acetate switch.
Microbiol Mol Biol Rev: 2005, 69(1);12-50
[PubMed:15755952] [WorldCat.org] [DOI] (P p)

Original publications

Additional publications: PubMed

V K Morya, Varun Dewaker, Eun-Ki Kim
In silico study and validation of phosphotransacetylase (PTA) as a putative drug target for Staphylococcus aureus by homology-based modelling and virtual screening.
Appl Biochem Biotechnol: 2012, 168(7);1792-805
[PubMed:23054816] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Qian Steven Xu, Jarmila Jancarik, Yun Lou, Kate Kuznetsova, Alexander F Yakunin, Hisao Yokota, Paul Adams, Rosalind Kim, Sung-Hou Kim
Crystal structures of a phosphotransacetylase from Bacillus subtilis and its complex with acetyl phosphate.
J Struct Funct Genomics: 2005, 6(4);269-79
[PubMed:16283428] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

E Presecan-Siedel, A Galinier, R Longin, J Deutscher, A Danchin, P Glaser, I Martin-Verstraete
Catabolite regulation of the pta gene as part of carbon flow pathways in Bacillus subtilis.
J Bacteriol: 1999, 181(22);6889-97
[PubMed:10559153] [WorldCat.org] [DOI] (P p)

B S Shin, S K Choi, S H Park
Regulation of the Bacillus subtilis phosphotransacetylase gene.
J Biochem: 1999, 126(2);333-9
[PubMed:10423526] [WorldCat.org] [DOI] (P p)