PonA

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Gene name ponA
Synonyms
Essential no
Product penicillin-binding protein 1A/1B
Function bifunctional glucosyltransferase/ transpeptidase
Gene expression levels in SubtiExpress: ponA
Interactions involving this protein in SubtInteract: PonA
Metabolic function and regulation of this protein in SubtiPathways:
PonA
MW, pI 99 kDa, 4.752
Gene length, protein length 2742 bp, 914 aa
Immediate neighbours recU, ypoC
Sequences Protein DNA DNA_with_flanks
Genetic context
PonA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PonA expression.png















Categories containing this gene/protein

cell wall synthesis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

  • Locus tag: BSU22320

Phenotypes of a mutant

  • prevents bulging of the cells when grown at low Mg(2+) concentrations, suppresses the lethal effect of a mreB mutation PubMed
  • deletion of ponA restores growth and normal shape of a yvcK mutant on gluconeogenic carbon sources PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: constitutive during vegetative growth PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
  • Antibody:

Labs working on this gene/protein

Jeff Errington, Newcastle University, UK homepage

Your additional remarks

References

Reviews

Johann Mignolet, Patrick H Viollier
A sweet twist gets Bacillus into shape.
Mol Microbiol: 2011, 80(2);283-5
[PubMed:21371139] [WorldCat.org] [DOI] (I p)

Original publications