YaaT

• Description: control of the phosphorelay, required for the achieving a sufficient level of Spo0A-P for sporulation initiation
  
  

Gene name	yaaT
Synonyms
Essential	no
Product	part of the YaaT-YmcA-YlbF complex
Function	regulation of sporulation initiation
Gene expression levels in SubtiExpress: yaaT
Interactions involving this protein in SubtInteract: YaaT
MW, pI	31 kDa, 4.838
Gene length, protein length	825 bp, 275 aa
Immediate neighbours	holB, yabA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphorelay, membrane proteins

The gene

Basic information

• Locus tag: BSU00320

Phenotypes of a mutant

• block of sporulation at stage 0 PubMed
• strongly reduced genetic competence, strongly reduced sporulation PubMed

Database entries

• BsubCyc: BSU00320

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • YmcA-YlbF-YaaT, this complex stimulates Spo0A phosphorylation in the phosphorelay PubMed

• Localization:
  • peripheral membrane, as well as to the septum, during sporulation PubMed

Database entries

• BsubCyc: BSU00320

• Structure:

• UniProt: P37541

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yaaQ-yaaR-holB-yaaT-yabA-yabB-yazA-yabC PubMed

• Expression browser: yaaT PubMed

• Sigma factor:

• Regulation:
  • expressed during growth and the transition phase, expression is erduced in stationary phase PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 116 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 172 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Valerie J Carabetta, Andrew W Tanner, Todd M Greco, Melissa Defrancesco, Ileana M Cristea, David Dubnau
A complex of YlbF, YmcA and YaaT regulates sporulation, competence and biofilm formation by accelerating the phosphorylation of Spo0A.
Mol Microbiol: 2013, 88(2);283-300
[PubMed:23490197] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Shigeo Hosoya, Kei Asai, Naotake Ogasawara, Michio Takeuchi, Tsutomu Sato
Mutation in yaaT leads to significant inhibition of phosphorelay during sporulation in Bacillus subtilis.
J Bacteriol: 2002, 184(20);5545-53
[PubMed:12270811] [WorldCat.org] [DOI] (P p)