MtlR

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  • Description: transcriptional activator of the mtlA-mtlF-mtlD operon

Gene name mtlR
Synonyms ydaA
Essential no
Product transcriptional activator, PRD-type
Function regulation of mannitol utilization
Gene expression levels in SubtiExpress: mtlR
Interactions involving this protein in SubtInteract: MtlR
Metabolic function and regulation of this protein in SubtiPathways:
mtlR
MW, pI 78 kDa, 5.313
Gene length, protein length 2082 bp, 694 aa
Immediate neighbours ycsN, ydaB
Sequences Protein DNA DNA_with_flanks
Genetic context
MtlR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MtlR expression.png















Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

CcpA regulon

The MtlR regulon:

The gene

Basic information

  • Locus tag: BSU04160

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: N-terminal DNA-binding domains, two PTS-regulation domains (PRD1 and PRD2), EIIB (Gat)-like domain, EIIA (Mtl)-like domain
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • activity is stimulated by PtsH-dependent phosphorylation in PRD2 (mechnism of carbon catabolite repression) PubMed
    • activity is inhibited by MtlF-dependent phosphorylation in the EIIB(Gat)-like domain on Cys-419 (this prevents activity in the absence of mannitol and allows induction in presence of mannitol) PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Philippe Joyet, Houda Bouraoui, Francine Moussan Désirée Aké, Meriem Derkaoui, Arthur Constant Zébré, Thanh Nguyen Cao, Magali Ventroux, Sylvie Nessler, Marie-Françoise Noirot-Gros, Josef Deutscher, Eliane Milohanic
Transcription regulators controlled by interaction with enzyme IIB components of the phosphoenolpyruvate: sugar phosphotransferase system.
Biochim Biophys Acta: 2013, 1834(7);1415-24
[PubMed:23318733] [WorldCat.org] [DOI] (P p)

J Stülke, M Arnaud, G Rapoport, I Martin-Verstraete
PRD--a protein domain involved in PTS-dependent induction and carbon catabolite repression of catabolic operons in bacteria.
Mol Microbiol: 1998, 28(5);865-74
[PubMed:9663674] [WorldCat.org] [DOI] (P p)


Original publications

Houda Bouraoui, Magali Ventroux, Marie-Françoise Noirot-Gros, Josef Deutscher, Philippe Joyet
Membrane sequestration by the EIIB domain of the mannitol permease MtlA activates the Bacillus subtilis mtl operon regulator MtlR.
Mol Microbiol: 2013, 87(4);789-801
[PubMed:23279188] [WorldCat.org] [DOI] (I p)

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

Kambiz Morabbi Heravi, Marian Wenzel, Josef Altenbuchner
Regulation of mtl operon promoter of Bacillus subtilis: requirements of its use in expression vectors.
Microb Cell Fact: 2011, 10;83
[PubMed:22014119] [WorldCat.org] [DOI] (I e)

Philippe Joyet, Meriem Derkaoui, Sandrine Poncet, Josef Deutscher
Control of Bacillus subtilis mtl operon expression by complex phosphorylation-dependent regulation of the transcriptional activator MtlR.
Mol Microbiol: 2010, 76(5);1279-94
[PubMed:20444094] [WorldCat.org] [DOI] (I p)

Shouji Watanabe, Miyuki Hamano, Hiroshi Kakeshita, Keigo Bunai, Shigeo Tojo, Hirotake Yamaguchi, Yasutaro Fujita, Sui-Lam Wong, Kunio Yamane
Mannitol-1-phosphate dehydrogenase (MtlD) is required for mannitol and glucitol assimilation in Bacillus subtilis: possible cooperation of mtl and gut operons.
J Bacteriol: 2003, 185(16);4816-24
[PubMed:12897001] [WorldCat.org] [DOI] (P p)

S A Henstra, R H Duurkens, G T Robillard
Multiple phosphorylation events regulate the activity of the mannitol transcriptional regulator MtlR of the Bacillus stearothermophilus phosphoenolpyruvate-dependent mannitol phosphotransferase system.
J Biol Chem: 2000, 275(10);7037-44
[PubMed:10702268] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

S A Henstra, M Tuinhof, R H Duurkens, G T Robillard
The Bacillus stearothermophilus mannitol regulator, MtlR, of the phosphotransferase system. A DNA-binding protein, regulated by HPr and iicbmtl-dependent phosphorylation.
J Biol Chem: 1999, 274(8);4754-63
[PubMed:9988713] [WorldCat.org] [DOI] (P p)