Scr

• Description: 4.5 S RNA
  
  

Gene name	scr
Synonyms
Essential	yes
Product	small cytoplasmic RNA (scRNA, 4.5S RNA), signal recognition particle-like (SRP) component
Function	presecretory protein translocation
Interactions involving this RNA in SubtInteract: Scr
Immediate neighbours	yaaJ, dnaX
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

protein secretion, essential genes, ncRNA

The gene

Basic information

• Locus tag:

Phenotypes of a mutant

• the gene is essential PubMed

Database entries

• BsubCyc: BSU_misc_RNA_2

• DBTBS entry: no entry

• SubtiList entry: [1]

• Structure:

The RNA

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

Extended information on the RNA

• Kinetic information

• Interactions:
  • Scr-Ffh in the signal recognition particle

• Localization

Database entries

• BsubCyc: BSU_misc_RNA_2

• Structure: 4WFL PubMed

Expression and regulation

• Operon: yaaJ-scr, scr

• Sigma factor: SigA (both promoters)

• Regulation:

• Regulatory mechanism:

• Additional information: transcribed as 354 nt precursor, cleaved at the 5' end by RNase III and at the 3' site by RNase J1 to yield a 275 nt molecule, final trimming to 271 nt sc RNA by RNase PH PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

References

Reviews

Zhen Kang, Sen Yang, Guocheng Du, Jian Chen
Molecular engineering of secretory machinery components for high-level secretion of proteins in Bacillus species.
J Ind Microbiol Biotechnol: 2014, 41(11);1599-607
[PubMed:25212246] [WorldCat.org] [DOI] (I p)