RadA

From SubtiWiki
Revision as of 10:36, 10 August 2015 by Jstuelk (talk | contribs)
Jump to: navigation, search
  • Description: 6-O-methylguanine-DNA methyltransferase, negative effector of DisA activity, participates in the stabilization and/or processing of holliday junction intermediates

Gene name radA
Synonyms sms, yacJ
Essential no
Product 6-O-methylguanine-DNA methyltransferase
Function homologous recombination , control of c-di-AMP formation
Gene expression levels in SubtiExpress: radA
Interactions involving this protein in SubtInteract: RadA
Metabolic function and regulation of this protein in SubtiPathways:
radA
MW, pI 49 kDa, 8.128
Gene length, protein length 1374 bp, 458 aa
Immediate neighbours clpC, disA
Sequences Protein DNA DNA_with_flanks
Genetic context
RadA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RadA expression.png















Categories containing this gene/protein

metabolism of signalling nucleotides, DNA repair/ recombination, general stress proteins (controlled by SigB), heat shock proteins

This gene is a member of the following regulons

CtsR regulon, SigB regulon

The gene

Basic information

  • Locus tag: BSU00870

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: RadA subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism: CtsR: transcription repression PubMed
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Deani L Cooper, Daniel C Boyle, Susan T Lovett
Genetic analysis of Escherichia coli RadA: functional motifs and genetic interactions.
Mol Microbiol: 2015, 95(5);769-79
[PubMed:25484163] [WorldCat.org] [DOI] (I p)

Lei Zhang, Zheng-Guo He
Radiation-sensitive gene A (RadA) targets DisA, DNA integrity scanning protein A, to negatively affect cyclic Di-AMP synthesis activity in Mycobacterium smegmatis.
J Biol Chem: 2013, 288(31);22426-36
[PubMed:23760274] [WorldCat.org] [DOI] (I p)

Begoña Carrasco, M Castillo Cozar, Rudi Lurz, Juan C Alonso, Silvia Ayora
Genetic recombination in Bacillus subtilis 168: contribution of Holliday junction processing functions in chromosome segregation.
J Bacteriol: 2004, 186(17);5557-66
[PubMed:15317759] [WorldCat.org] [DOI] (P p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

I Derré, G Rapoport, T Msadek
CtsR, a novel regulator of stress and heat shock response, controls clp and molecular chaperone gene expression in gram-positive bacteria.
Mol Microbiol: 1999, 31(1);117-31
[PubMed:9987115] [WorldCat.org] [DOI] (P p)

E Krüger, T Msadek, M Hecker
Alternate promoters direct stress-induced transcription of the Bacillus subtilis clpC operon.
Mol Microbiol: 1996, 20(4);713-23
[PubMed:8793870] [WorldCat.org] [DOI] (P p)