OpuAC

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  • Description: glycine betaine ABC transporter (binding protein)

Gene name opuAC
Synonyms
Essential no
Product glycine betaine ABC transporter (binding protein)
Function compatible solute transport
MW, pI 32 kDa, 8.015
Gene length, protein length 879 bp, 293 aa
Immediate neighbours opuAB, amhX
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
OpuAC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure: 2B4L (complex with glycine betaine), 3CHG (in complex with DMSA)
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

Your additional remarks

References

  1. Smits SH, Höing M, Lecher J, Jebbar M, Schmitt L, Bremer E. (2008) The compatible-solute-binding protein OpuAC from Bacillus subtilis: ligand binding, site-directed mutagenesis, and crystallographic studies. J Bacteriol. Aug;190(16): 5663-71. PubMed
  2. Kempf B, Bremer E. (1995) OpuA, an osmotically regulated binding protein-dependent transport system for the osmoprotectant glycine betaine in Bacillus subtilis. J Biol Chem. Jul 14;270(28): 16701-13. PubMed
  3. Horn C, Bremer E, Schmitt L. (2005) Functional overexpression and in vitro re-association of OpuA, an osmotically regulated ABC-transport complex from Bacillus subtilis. FEBS Lett. Oct 24;579(25): 5765-8. PubMed
  4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed