PurR

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  • Description: transcription repressor of the pur operon

Gene name purR
Synonyms yabI
Essential no
Product transcription repressor of the pur operon
Function regulation of purine biosynthesis
MW, pI 31 kDa, 5.771
Gene length, protein length 855 bp, 285 aa
Immediate neighbours ispE, yabJ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PurR context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Biological activity: transcriptional repressor of purine biosynthetic operons
  • Protein family: purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: PRPP acts as inducer, binding results in release of PurR from the operator PubMed
  • Localization:

Database entries

  • E.C. number:

Additional information

requires YabJ for in vivo activity

Expression and regulation

  • Regulation: negative autoregulation, induction in the presence of PRPP PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Shuobo Shi, Tao Chen, Zhigang Zhang, Xun Chen, Xueming Zhao
Transcriptome analysis guided metabolic engineering of Bacillus subtilis for riboflavin production.
Metab Eng: 2009, 11(4-5);243-52
[PubMed:19446032] [WorldCat.org] [DOI] (I p)

Aloke Kumar Bera, Jianghai Zhu, Howard Zalkin, Janet L Smith
Functional dissection of the Bacillus subtilis pur operator site.
J Bacteriol: 2003, 185(14);4099-109
[PubMed:12837784] [WorldCat.org] [DOI] (P p)

Sangita C Sinha, Joseph Krahn, Byung Sik Shin, Diana R Tomchick, Howard Zalkin, Janet L Smith
The purine repressor of Bacillus subtilis: a novel combination of domains adapted for transcription regulation.
J Bacteriol: 2003, 185(14);4087-98
[PubMed:12837783] [WorldCat.org] [DOI] (P p)

M Weng, H Zalkin
Mutations in the Bacillus subtilis purine repressor that perturb PRPP effector function in vitro and in vivo.
Curr Microbiol: 2000, 41(1);56-9
[PubMed:10919400] [WorldCat.org] [DOI] (P p)

M Weng, P L Nagy, H Zalkin
Identification of the Bacillus subtilis pur operon repressor.
Proc Natl Acad Sci U S A: 1995, 92(16);7455-9
[PubMed:7638212] [WorldCat.org] [DOI] (P p)


  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed