CsaA

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  • Description: molecular chaperone involved in protein secretion

Gene name csaA
Synonyms
Essential no
Product molecular chaperone
Function protein secretion
MW, pI 11 kDa, 8.691
Gene length, protein length 330 bp, 110 aa
Immediate neighbours bsrF, yobQ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CsaA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU19040

Phenotypes of a mutant

From unsuccessful trials to knock out the gene, it was concluded that csaA apparently is essential. However, in a conditional mutant (Pspac-csaA) depletion of the gene product is not lethal. However, the secretion of some proteins including YvaY was reported to be affected unter such conditions.

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Secretion associated chaperone. Assumed functional analogue of E. coli SecB
  • Protein family: FIG1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: CsaA contains a tRNA binding domain.
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Yuliya A Shapova, Mark Paetzel
Crystallographic analysis of Bacillus subtilis CsaA.
Acta Crystallogr D Biol Crystallogr: 2007, 63(Pt 4);478-85
[PubMed:17372352] [WorldCat.org] [DOI] (P p)

Dirk Linde, Rudolf Volkmer-Engert, Sandra Schreiber, Jörg P Müller
Interaction of the Bacillus subtilis chaperone CsaA with the secretory protein YvaY.
FEMS Microbiol Lett: 2003, 226(1);93-100
[PubMed:13129613] [WorldCat.org] [DOI] (P p)

J P Müller, J Ozegowski, S Vettermann, J Swaving, K H Van Wely, A J Driessen
Interaction of Bacillus subtilis CsaA with SecA and precursor proteins.
Biochem J: 2000, 348 Pt 2(Pt 2);367-73
[PubMed:10816431] [WorldCat.org] (P p)

Jörg P Müller, Sierd Bron, Gerard Venema, Jan Maarten van Dijl
Chaperone-like activities of the CsaA protein of Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 1);77-88
[PubMed:10658654] [WorldCat.org] [DOI] (P p)


  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed