AnsZ

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  • Description: asparaginase

Gene name ansZ
Synonyms yccC
Essential no
Product asparaginase
Function asparagine utilization
Metabolic function and regulation of this protein in SubtiPathways:
Asp, Asn
MW, pI 39 kDa, 6.15
Gene length, protein length 1125 bp, 375 aa
Immediate neighbours lmrA, lip
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YccC context.gif
This image was kindly provided by SubtiList




The gene

Basic information

  • Locus tag: BSU02690

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-asparagine + H2O = L-aspartate + NH3 (according to Swiss-Prot)
  • Protein family: asparaginase 1 family (according to Swiss-Prot)
  • Paralogous protein(s): AnsA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon: ansZ
  • Regulation: repressed by CodY PubMed
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References