speA

168

[metabolite|arginine] decarboxylase, required for [wiki|biofilm formation]

locus: BSU_14630

Molecular weight: 53.42 kDa

pI: 5.08

Protein length: 490 aa

Gene length: 1470 bp

function: [metabolite|spermidine], polyamine biosynthesis

product: [metabolite|arginine] decarboxylase

essential: no

ec: 4.1.1.19

synonyms: speA, cad

List of homologs in different organisms, belongs to COG1982 (Galperin et al., 2021)

Gene

Coordinates: 1,534,279 → 1,535,751

Phenotypes of a mutant

• no [wiki|biofilm formation] [Pubmed|24529384,20876533]

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The protein

Catalyzed reaction/ biological activity

• H⁺ + [metabolite|L-arginine] --> [metabolite|agmatine] + CO₂ (according to UniProt)

Protein family

• Orn/Lys/Arg decarboxylase class-I family (together with [protein|EEF3E572ED2F23581EB9B48D16B3112B886F7975|yaaO]) (according to UniProt)

[wiki|Cofactors]

• PLP (according to UniProt)

Structure

• [PDB|2X3L] (from Staphylococcus aureus, 28% identity) [pubmed|20419351]
• [AF|P21885]

Paralogous protein(s)

• [protein|EEF3E572ED2F23581EB9B48D16B3112B886F7975|yaaO]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|03E5E41FD7ADA4E9CB70E312FA5F163331C92695|speA]

  description: [pubmed|22383849]

Biological materials

Mutant

• CS210 (''[gene|03E5E41FD7ADA4E9CB70E312FA5F163331C92695|speA]''::''aphA3'', available in [wiki|Colin Harwood]'s and [wiki|Jörg Stülke]'s labs) [Pubmed|27197833]
• BKE14630 (Δ[gene|03E5E41FD7ADA4E9CB70E312FA5F163331C92695|speA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTGTTTTCCCACCTTTG,  downstream forward: _UP4_TAAAAAATAAAAAGCATGCG
• BKK14630 (Δ[gene|03E5E41FD7ADA4E9CB70E312FA5F163331C92695|speA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTGTTTTCCCACCTTTG,  downstream forward: _UP4_TAAAAAATAAAAAGCATGCG

References

• 24529384,19255484,9723923,20876533,27197833,20419351