Difference between revisions of "ConE"

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(Labs working on this gene/protein)
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Completely defective for conjugal transfer of ICE''Bs1''
+
Defective for conjugal transfer of ICE''Bs1''
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 16:09, 10 September 2009

  • Description: conjugation protein of the integrative and conjugative element ICEBs1

Gene name conE
Synonyms yddE
Essential no
Product conjugation protein
Function conjugative transfer of ICEBs1
MW, pI 96 kDa, 5.511
Gene length, protein length 2493 bp, 831 aa
Immediate neighbours yddD, yddF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YddE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU04940

Phenotypes of a mutant

Defective for conjugal transfer of ICEBs1

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: required for conjugative transfer of ICEBs1, putative ATPase PubMed
  • Protein family: HerA/FtsK Superfamily of ATPases including the well-studied proteins FtsK, SpoIIIE, VirB4, and VirD4
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: Located at the cell poles, in close association with the membrane

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Melanie Barker Berkmen, Suffolk University, Boston, MA

Your additional remarks

References

Melanie B Berkmen, Catherine A Lee, Emma-Kate Loveday, Alan D Grossman
Polar positioning of a conjugation protein from the integrative and conjugative element ICEBs1 of Bacillus subtilis.
J Bacteriol: 2010, 192(1);38-45
[PubMed:19734305] [WorldCat.org] [DOI] (I p)