Difference between revisions of "Crh"

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(Biological materials)
(Biological materials)
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* '''two-hybrid system:''' ''[[crh]]'', ''crh(Ser46Asp)'', ''crh(Ser46Ala)'' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Boris Görke| Görke]] lab
 
* '''two-hybrid system:''' ''[[crh]]'', ''crh(Ser46Asp)'', ''crh(Ser46Ala)'' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Boris Görke| Görke]] lab
  
* '''FLAG-tag construct:''' GP69 (spc, based on [[pGP1331]]), available in the [[Boris Görke]] and [[Stülke]] labs
+
* '''FLAG-tag construct:
 +
** GP69 (spc, based on [[pGP1331]]), available in the [[Boris Görke]] and [[Stülke]] labs
 +
** pGP1200 ''crh''(Ser46Ala) (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
  
 
* '''Antibody:''' available in [[Boris Görke| Görke]] lab
 
* '''Antibody:''' available in [[Boris Görke| Görke]] lab

Revision as of 08:58, 7 November 2011

  • Description: "Catabolite repression HPr-like protein", control of flux through the harmful methylglyoxal pathway, minor cofactor of the CcpA transcription factor

Gene name crh
Synonyms yvcM
Essential no
Product catabolite repression HPr-like protein
Function control of carbon flux
Interactions involving this protein in SubtInteract: Crh
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 9,2 kDa, 4.70
Gene length, protein length 255 bp, 85 amino acids
Immediate neighbours yvcN, yvcL
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Crh context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU34740

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: HPr family
  • Paralogous protein(s): HPr

Extended information on the protein

  • Kinetic information:
  • Domains: HPr domain (1–85)
  • Modification: phosphorylation on Ser46 by HprK PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries


  • KEGG entry: [2]

Additional information

Crh does not possess the phosphorylation site used for PTS phosphotransfer (His-15 in PtsH), it can only be phosphorylated on Ser-46

Expression and regulation

  • Regulation: very weak stimuation of expression by citrate and succinate PubMed
  • Regulatory mechanism:
  • Additional information: Crh is weakly expressed. This results in part from a poorly conserved ribosomal binding site of the mRNA. PubMed

Biological materials

  • Mutant: GP860 (aphA3), QB7097 (spc), available in Stülke lab
  • Expression vector:
    • pGP641 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
    • pGP734 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Stülke lab
  • lacZ fusion: see yvcI
  • GFP fusion:
  • two-hybrid system: crh, crh(Ser46Asp), crh(Ser46Ala) B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Anne Galinier, University of Marseille, France

Wolfgang Hillen, Erlangen University, Germany Homepage

Richard Brennan, Houston, Texas, USA Homepage

Your additional remarks

References