Difference between revisions of "DeaD"

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(Extended information on the protein)
(Extended information on the protein)
Line 63: Line 63:
  
 
* '''Domains:'''  
 
* '''Domains:'''  
Helicase domain 1: AS 1-203
+
**Helicase domain 1: AS 1-203
Linker region: AS 204-212
+
**Linker region: AS 204-212
Helicase domain 2: AS 207-368
+
**Helicase domain 2: AS 207-368
RNA-binding domain: AS 404-479
+
**RNA-binding domain: AS 404-479
  
 
* '''Modification:'''
 
* '''Modification:'''

Revision as of 16:32, 19 February 2009

  • Description: DEAD-box RNA helicase

Gene name deaD
Synonyms yxiN
Essential no
Product ATP-dependent RNA helicase
Function RNA helicase
MW, pI 53 kDa, 7.706
Gene length, protein length 1437 bp, 479 aa
Immediate neighbours
Gene sequence (+200bp) Protein sequence
Genetic context
DeaD context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: RNA helicase
  • Protein family: DEAD-box RNA helicase

Extended information on the protein

  • Kinetic information:
  • Domains:
    • Helicase domain 1: AS 1-203
    • Linker region: AS 204-212
    • Helicase domain 2: AS 207-368
    • RNA-binding domain: AS 404-479
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1120 (spc), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed