Difference between revisions of "Easter egg"

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* '''Description:''' inhibitor of [[AbrB]] activity<br/><br/>
 
  
{| align="right" border="1" cellpadding="2"
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|''abbA ''
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykzF''
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Product''' || anti-repressor
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || inhibition of [[AbrB]]
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 7 kDa, 4.642 
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 195 bp, 65 aa
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykuK]]'', ''[[ykuL]]''
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13285&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ykzF_context.gif]]
 
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|}
 
  
__TOC__
+
[[File:Example.jpg]]
 
 
<br/><br/>
 
 
 
=The gene=
 
 
 
=== Basic information ===
 
 
 
* '''Locus tag:''' BSU14120
 
 
 
===Phenotypes of a mutant ===
 
 
 
=== Database entries ===
 
 
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ykuJK-ykzF-ykuL-ccpC.html]
 
 
 
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13334]
 
 
 
=== Additional information===
 
 
 
 
 
 
 
= Categories containing this gene/protein =
 
{{SubtiWiki category|[[transcription factors and their control]]}}
 
=The protein=
 
 
 
=== Basic information/ Evolution ===
 
 
 
* '''Catalyzed reaction/ biological activity:''' inhibition of AbrB activity [http://www.ncbi.nlm.nih.gov/sites/entrez/18840696 PubMed]
 
 
 
* '''Protein family:'''
 
 
 
* '''Paralogous protein(s):'''
 
 
 
=== Extended information on the protein ===
 
 
 
* '''Kinetic information:'''
 
 
 
* '''Domains:'''
 
 
 
* '''Modification:'''
 
 
 
* '''Cofactor(s):'''
 
 
 
* '''Effectors of protein activity:'''
 
 
 
* '''Interactions:''' [[AbbA]]-[[AbrB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18840696 PubMed]
 
 
 
* '''Localization:'''
 
 
 
=== Database entries ===
 
 
 
* '''Structure:'''
 
 
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O31697 O31697]
 
 
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU14120]
 
 
 
* '''E.C. number:'''
 
 
 
=== Additional information===
 
 
 
=Expression and regulation=
 
 
 
* '''Operon:''' ''[[ykuJ]]-[[ykuK]]-[[abbA]]-[[ykuL]]-[[ccpC]]'' {{PubMed|16395550}}
 
 
 
* '''Sigma factor:'''
 
 
 
* '''Regulation:'''
 
** repressed by glucose (4.3-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
 
** expressed under conditions that trigger sporulation ([[Spo0A]]) {{PubMed|18840696,14651647}}
 
 
 
* '''Regulatory mechanism:'''
 
** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12850135 PubMed]
 
** [[Spo0A]]: transcription activation by [[Spo0A]]~P {{PubMed|18840696,14651647}}
 
 
 
* '''Additional information:'''
 
 
 
=Biological materials =
 
 
 
* '''Mutant:''' GP214 (deletion of ''[[ykuJ]]-[[ykuK]]-[[abbA]]-[[ykuL]]'', replaced by ''aphA3''), available in the [[Stülke]] lab
 
 
 
* '''Expression vector:'''
 
       
 
* '''lacZ fusion:''' pGP708 (in [[pAC5]]), available in [[Stülke]] lab, pGP712 (in [[pAC6]]), available in [[Stülke]] lab
 
 
 
* '''GFP fusion:'''
 
 
 
* '''two-hybrid system:'''
 
 
 
* '''Antibody:'''
 
 
 
=Labs working on this gene/protein=
 
 
 
[[Richard Losick]], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]
 
 
 
=Your additional remarks=
 
 
 
=References=
 
 
 
<pubmed>,18840696,14651647,12850135 18840696 12850135,16395550 </pubmed>
 
 
 
[[Category:Protein-coding genes]]
 

Latest revision as of 16:46, 11 March 2012


Example.jpg