GapB

• Description: glyceraldehyde-3-phosphate dehydrogenase catalyses the syntheseis of 1,3 Bisphosphoglycerat, phosphorylation of ADP and reduction of NAD
  
  

Gene name	gapB
Synonyms
Essential	no
Product	glyceraldehyde-3-phosphate dehydrogenase2
Function	syntheseis of 1,3 Bisphosphoglycerat
MW, pI	37,3 kDa, 6.47
Gene length, protein length	1020 bp, 340 amino acids
Immediate neighbours	ytcD, speD
Gene sequence (+200bp)	Protein sequence
Genetic context File:GenE context.gif

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-glyceraldehyde 3-phosphate + phosphate + NAD(P)(+) = 3-phospho-D-glyceroyl phosphate + NAD(P)H.

• Protein family: glyceraldehyde-3-phosphate dehydrogenase family

• Paralogous protein(s): GapA

Extended information on the protein

• Kinetic information:

• Domains:
  • Nucleotid binding Domain (12-13)
  • 2x Glyceraldehyde 3-phosphate binding Domain (151-153) & (210-211)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: Cytoplasm

Database entries

• Structure:

• Swiss prot entry: [3]

• KEGG entry: [4]

• E.C. number: [5]

Expression and regulation

• Operon:

• Sigma factor: SigA

• Regulation: repressed (70-times) by Glc, repressor CcpN

• Regulatory mechanism: transcription repression

• Additional information:

References

1. Meile JC, Wu LJ, Ehrlich SD (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6(7): 2135-46. PubMed
2. Servant et al. (2005) CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes. Mol. Microbiol. 55: 1435-1451. PubMed
3. Tännler et al. (2008) CcpN controls central carbon fluxes in Bacillus subtilis. J. Bacteriol. 190: 6178-6187. PubMed