Difference between revisions of "GltC"

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(Biological Materials)
(Biological Materials)
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=Biological Materials =
 
=Biological Materials =
  
* '''Mutant:'''
+
* ''Mutant:'', ''Expression vector''
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 18:38, 10 December 2008

  • Synonyms:
  • Description: Transcriptional activator of the gltAB operon. Activates expression of the operon in the absence of arginine.

The gene

Basic information

  • Coordinates:
  • Gene length:

Database entries

  • DBTBS entry:

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcription activation of the gltAB operon
  • Protein family: LysR-type transcription regulator
  • Paralogous protein(s): none, but there are 19 members of the LysR family in B. subtilis

Database entries

  • Structure
  • Swiss prot entry
  • KEGG entry

Extended information on the protein

  • Kinetic information
  • Domains DNA-binding helix-turn-helix motif: AA 18 ... 37
  • Modification
  • Effectors of protein activity:2-oxoglutarate
  • Interactions:
    • GltC-RocG, This interaction takes place in the presence of glutamate. It prevents the transcription activation of the gltAB operon. Note that RocG expression is strongly regulated. <ref>Commichau et al. (2007)</ref>
  • Localization

Expression and Regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:

Biological Materials

  • Mutant:, Expression vector
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • Antibody: available in Stülke lab

Phenotypes of a Mutant

Additional remarks

  • Labs working on this gene/protein:

Linc Sonenshein, Tufts University, Boston, MA, USA [1]

Jörg Stülke, University of Göttingen, Germany [2]

References